Biotin and protein synthesis in rat liver.

Biotin administration to biotin-deficient rats enhanced protein synthesis. Time sequence studies indicated that RNA synthesis was stimulated as early as 1 hour after biotin administration to the deficient rat. The synthesis of RNA of liver was stimulated by biotin administration. Nuclear RNA isolated from biotin-injected rats was more active in a protein-synthesizing system in vitro than a similar preparation from the deficient rat liver. It is suggested that biotin has a role in the synthesis of RNA.